Bioinformatics CNV Detection with Random Forest Model
A package for automated quantification of non-orthogonal, multiplexed ddPCR data
Fortify your ddPCR analyses for low-frequency variant detection
vflank is the variant-aware, optionally patient-specific, masked-flank front-end of a ddPCR assay-design pipeline. It extracts the sequence flanking each variant and masks positions that would compromise a primer/probe, then emits clean targets in designer-native formats.
Batch in silico PCR assay validation against genome assemblies. BLAST-based sensitivity/specificity screening for ddPCR/qPCR/SYBR primers and probes, with a companion tool for clade-specific target discovery.
An interactive, web based GUI for the ddPCRclust algorithm. It was developed using the web application framework Shiny.
A single-page web front-end for vflank — paste, upload, or type a handful of variants and get back masked ddPCR target sequences (and optional Primer3 input), with no install and no patient data leaving the browser.
Digital PCR Calculator: Ligant Bench Tools. Source for local use. Research use only. Not qualified for GxP decision-making. Hosted at benchtools.ligant.ai/digital-pcr-calculator/
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